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«Nanotechnology Research and Practice» – international scientific Journal.

E-ISSN 2413-7227

Publication frequency – once a year.
Issued from 2014.

3 September 30, 2015

1. Ignat Ignatov, Oleg Mosin
Structural Models of Water and Ice Regarding the Energy of Hydrogen Bonding

Nanotechnology Research and Practice, 2015, Vol.(7), Is. 3, pp. 96-118.
DOI: 10.13187/ejnr.2015.7.96CrossRef

In this review it is reported about the research on the structure of water and ice and intermolecular water cyclic associates (clusters) with general formula (Н2О)n and their charged ionic clusters [(Н2О)n]+ and [(Н2О)n]- by means of computer modelling and spectroscopy methods as 1Н-NMR, IR-spectroscopy, DNES, EXAFS-spectroscopy, X-Ray and neurons diffraction. The computer calculation of polyhedral nanoclusters (Н2О)n, where n = 3–20 are carried out. Based on this data the main structural mathematical models describing water structure (quasicrystalline, continious, fractal, fractal-clathrate) have been examined and some important physical characteristics were obtained. The average energy of hydrogen bonding (EH…O) between Н2О molecules in the process of cluster formation was measured by the DNES method compiles –0,1067±0,0011 eV. It was also shown that water clusters formed from D2О were more stable, than those ones from Н2О due to isotopic effects of deuterium.

URL: http://ejournal13.com/journals_n/1448281409.pdf
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2. Oleg Mosin , Ignat Ignatov
Introduction of Deuterated Aromatic Amino Acids  [2,3,4,5,6-2H5]phenylalanine, [3,5-2H2]tyrosine and [2,4,5,6,7-2H5]tryptophan into a Molecule of Photochrome Trans-membrane Protein Bacteriorhodopsin

Nanotechnology Research and Practice, 2015, Vol.(7), Is. 3, pp. 119-136.
DOI: 10.13187/ejnr.2015.7.119CrossRef

It was carried out the introduction of functionally important deuterated aromatic amino acids  [2,3,4,5,6-2H5]phenylalanine, [3,5-2H2]tyrosine and [2,4,5,6,7-2H5]tryptophan into a molecule of photochrome trans-membrane protein bacteriorhodopsin, synthesized by a photo-organotrophic halobacterium Halobacterium halobium ET 1001. The deuterated protein (output 810 mg) was isolated from purple membranes by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, with the subsequent solubilization of final product with 0,5 % (w/v) SDS-Na and fractionation by methanol, gel filtration chromatography on Sephadex G-200, reverse-phase HPLC and EI impact mass-spectrometry of methyl esters of N-Dns-[2H]derivatives of amino acids. Deuterium was detected in all residues of aromatic amino acids. However, the presence in the EI mass spectrum of the BR hydrolysate the peaks [M]+ of semi-deuterated analogues of aromatic amino acids  phenylalanine with [M]+ at m/z = 413418, tyrosine  with [M]+ at m/z = 428430 and tryptophan  with [M]+ at m/z = 453457 with different levels of contributions to the deuterium enrichment of molecules testifies about the conservation of the minor pathways of biosynthesis of aromatic amino acids de novo.

URL: http://ejournal13.com/journals_n/1448281559.pdf
Number of views: 1555      Download in PDF

full number
URL: http://ejournal13.com/journals_n/1448283549.pdf
Number of views: 1594      Download in PDF

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