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«Nanotechnology Research and Practice» – international scientific Journal.

E-ISSN 2413-7227

Publication frequency – once a year.
Issued from 2014.

3 September 30, 2015


1. Ignat Ignatov, Oleg Mosin
Structural Models of Water and Ice Regarding the Energy of Hydrogen Bonding

Nanotechnology Research and Practice, 2015, Vol.(7), Is. 3, pp. 96-118.
DOI: 10.13187/ejnr.2015.7.96CrossRef

Abstract:
In this review it is reported about the research on the structure of water and ice and intermolecular water cyclic associates (clusters) with general formula (Н2О)n and their charged ionic clusters [(Н2О)n]+ and [(Н2О)n]- by means of computer modelling and spectroscopy methods as 1Н-NMR, IR-spectroscopy, DNES, EXAFS-spectroscopy, X-Ray and neurons diffraction. The computer calculation of polyhedral nanoclusters (Н2О)n, where n = 3–20 are carried out. Based on this data the main structural mathematical models describing water structure (quasicrystalline, continious, fractal, fractal-clathrate) have been examined and some important physical characteristics were obtained. The average energy of hydrogen bonding (EH…O) between Н2О molecules in the process of cluster formation was measured by the DNES method compiles –0,1067±0,0011 eV. It was also shown that water clusters formed from D2О were more stable, than those ones from Н2О due to isotopic effects of deuterium.

URL: http://ejournal13.com/journals_n/1448281409.pdf
Number of views: 1409      Download in PDF


2. Oleg Mosin , Ignat Ignatov
Introduction of Deuterated Aromatic Amino Acids  [2,3,4,5,6-2H5]phenylalanine, [3,5-2H2]tyrosine and [2,4,5,6,7-2H5]tryptophan into a Molecule of Photochrome Trans-membrane Protein Bacteriorhodopsin

Nanotechnology Research and Practice, 2015, Vol.(7), Is. 3, pp. 119-136.
DOI: 10.13187/ejnr.2015.7.119CrossRef

Abstract:
It was carried out the introduction of functionally important deuterated aromatic amino acids  [2,3,4,5,6-2H5]phenylalanine, [3,5-2H2]tyrosine and [2,4,5,6,7-2H5]tryptophan into a molecule of photochrome trans-membrane protein bacteriorhodopsin, synthesized by a photo-organotrophic halobacterium Halobacterium halobium ET 1001. The deuterated protein (output 810 mg) was isolated from purple membranes by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, with the subsequent solubilization of final product with 0,5 % (w/v) SDS-Na and fractionation by methanol, gel filtration chromatography on Sephadex G-200, reverse-phase HPLC and EI impact mass-spectrometry of methyl esters of N-Dns-[2H]derivatives of amino acids. Deuterium was detected in all residues of aromatic amino acids. However, the presence in the EI mass spectrum of the BR hydrolysate the peaks [M]+ of semi-deuterated analogues of aromatic amino acids  phenylalanine with [M]+ at m/z = 413418, tyrosine  with [M]+ at m/z = 428430 and tryptophan  with [M]+ at m/z = 453457 with different levels of contributions to the deuterium enrichment of molecules testifies about the conservation of the minor pathways of biosynthesis of aromatic amino acids de novo.

URL: http://ejournal13.com/journals_n/1448281559.pdf
Number of views: 1364      Download in PDF


3.
full number
URL: http://ejournal13.com/journals_n/1448283549.pdf
Number of views: 1409      Download in PDF





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