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«Nanotechnology Research and Practice» – international scientific Journal.

E-ISSN 2413-7227

Publication frequency – once a year.
Issued from 2014.

4 December 30, 2015

1. Ignat Ignatov, Oleg Mosin
The Electron Microscopy of Micro-Dispersed Colloid Silver Nanoparticles

Nanotechnology Research and Practice, 2015, Vol.(8), Is. 4, pp. 140-150.
DOI: 10.13187/ejnr.2015.8.140CrossRef

In this review article are studied the methods for preparation of micro dispersed colloid silver nanoparticles and the electron microscopy, as well as the scopes of their practical application in nano-industry, bionanotechnology and water processing and water purification techniques. The mechanisms of bactericidal action of colloid silver on a microbial cell are discussed from the point of view of absorptive, electrostatic, enzyme and mutagen theories. Silver exerts both bactericidal and bacteriostatic effect against more than 500 species of microorganisms. The bactericidal effect of silver is 1.500 times more than the same concentration of phenol and 3.5 times more than the action of mercuric chloride. It is shown that the effects of colloid silver are defined by the concentration, the sizes of micro dispersed nanoparticles and their stability in water solutions. The micro dispersed silver nanoparticles may be prepared by using various physical-chemical, biochemical and biotechnological methods.

URL: http://ejournal13.com/journals_n/1452700632.pdf
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2. Oleg Mosin, Ignat Ignatov
The Improved Method for Isolation of Photochrome Trans-membrane Protein Bacteriorhodopsin from Purple Membranes of Halobacterium Halobacterium Halobium ET 1001

Nanotechnology Research and Practice, 2015, Vol.(8), Is. 4, pp. 151-164.
DOI: 10.13187/ejnr.2015.8.151CrossRef

It was developed the improved method for isolation of photochrome trans-membraine protein bacteriorhodopsin (output – 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H+ and АТP. The protein was isolated from purple membranes of photo-organotrophic halobacterium Halobacterium halobium ET 1001 by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, solubilization with 0.5% (w/v) SDS-Na, fractionation by MeOH and column gel permeation chromatography (GPC) of the final protein on Sephadex G-200 with 0.1% (w/v) SDS-Na and 2.5 mM ETDA. The homogeneity of the isolated bacteriorhodopsin was proved by combination of preparative and analytical methods, including elecrtophoresis in 12.5% (w/v) PAAG with 0.1% (w/v) SDS-Na and regeneration of apomembranes with 13-trans-retinal.

URL: http://ejournal13.com/journals_n/1452700694.pdf
Number of views: 1360      Download in PDF

full number
URL: http://ejournal13.com/journals_n/1452700720.pdf
Number of views: 1350      Download in PDF

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